But not before fans had to prove themselves.
A poll of six cult shows was put on the TV Guide's website including: Supernatural, Chuck, Bones, Castle, How I Met Your Mother and Smallville.
After two weeks of voting and five million votes, it came down to the wire. For the last three days of voting, Supernatural and Chuck fans were battling across Tumblr and Twitter to see their favorite boys on the cover.
But the Sam and Dean girls won out! Don't miss this week's issue that hits newsstands on Thursday with the Winchester brothers on the cover!
Check out this video below to watch editor Debra Birnbaum unveil the gorgeous cover.
And check out this short video from Jared Padalecki, Jensen Ackles, Misha Collins and Jim Beaver to thank the fans for their votes.